Estrogenic Activity of Bauhinia racemosa Extract in Female Albino Rats: An Investigational Study

Objective: the objective was to evaluate the effect of petroleum ether, ethanol, and water extract of Bauhinia racemosa in female albino rats. Materials and Methods: Plant extracts were tested for their estrogenic activity using ovariectomized method at two dose level: 200 mg/kg and 400 mg/kg, respectively. Further, plant extracts and standard drug ethinyl estradiol (1 μg/kg) combination were tested for synergistic estrogenic activity. Results: In‐vivo investigation revealed that ethanol extract at dose of 200 mg/kg and 400 mg/kg shows significance increase 325.23 ± 5.23, 328.84 ± 4.56 and 3.252 ± 0.47, 3.288 ± 0.27 in uterine wet weight and uterine weight ratio, respectively, in female albino rat as compared to control and petroleum ether and water extracts. Simultaneous administration of standard drug ethinyl estradiol (1 μg/kg) with ethanol extract at dose of 200 mg/kg and 400 mg/kg potentiates (327.36 ± 5.78, 330.95 ± 6.21 and 3.273 ± 0.64, 3.309 ± 0.49 uterine wet weight and uterine weight ratio respectively in female albino rat) the estrogenic activity as compared to individual administration of ethanol extract as a synergistic effect. Conclusion: It was observed that ethanol extract of B. racemosa produced significance estrogenic activity.